FastQCFastQC Report
Thu 6 Aug 2026
lib_213544_sample_1703730_S33_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelib_213544_sample_1703730_S33_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25358188
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGATGATTATCGCGT2127280.8388927473840008TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGATGATTATCTCGT1252240.4938207729984493TruSeq Adapter, Index 20 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)